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rodA [2020-04-05 12:52:42]
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rodA [2020-04-05 12:52:42]

SEDS family peptidoglycan glycosyltransferase, control of cell shape and elongation
locus
BSU_38120
pI
8.76
mw
43.12 kDa
protein length
393 aa Sequence Blast
gene length
1182 bp Sequence Blast
function
control of cell shape and elongation
product
SEDS family peptidoglycan glycosyltransferase
essential
yes
synonyms
ywcF,ipa-42d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,912,332 → 3,913,513

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • CRISPRi knockdown mutant has a terminal phenotype of severe lysis [Pubmed|27238023]

    • The protein

    Catalyzed reaction/ biological activity

  • principal peptidoglycan glycosyltransferase of the [SW|elongasome] [Pubmed|27525505]
  • [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)

    • Protein family

  • [SW|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]
  • SEDS family (with [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW] and [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE], according to UniProt)

    • Paralogous protein(s)

  • [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE], [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW]

    • Structure

  • [PDB|6BAR] (from Thermus thermophilus, 26% identity) [pubmed|29590088]

    • [SW|Localization]

  • integral membrane protein [Pubmed|21636744]
  • forms circumferentially moving patches [Pubmed|21636744]
  • localization depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] [SW|26679002]

    • Expression and Regulation

    Operons

    genes
    [gene|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
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    Biological materials

    Mutant

  • xylose-inducible CRISPRi knockdown mutant BEC38120 is available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Daniel_R._Zeigler BGSC]

    • labs

  • [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
  • [SW|David Rudner], Harvard Medical School, MA, USA [http://rudnerlab.med.harvard.edu/ homepage]

    • References

    Reviews

  • 26679002,28975672

    • Original Publications

  • 27525505, 27238023, 9622350,21636744,21636745,17981970,28085152,29590088